PC software GfsWin V3.83
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PC software WinControl V3.39
Version 3.33 and higher is compatible with all first-generation fluorometers originally operated with WinControl-2 software.
PC software PamWin 4 03s
The latest generation of PamWin software was created for the new MULTI-COLOR-PAM-II unit, which enables time resolutions in the sub-microsecond range.
Note: From software version 3.20 on, the duration of saturation pulses/multiple turnover pulses (the “SP-Width” parameter) is adjusted in 10 ms increments. Earlier software versions use 100 ms increments. Therefore, check your SP-Width setting after installation of new PamWin-3 software.
Software LSA-2050 1.06 (Windows 10, 11 64 Bit)
PC software Bustool to download data from MONI-DA (MONI-DA/S) internal flash memory chip. EXE-FILE (2.2 MB)
PC software ImagingWinGigE V2.57q39
PC software ImagingWinGigE V2.56zn
EXE-File (45.8 MB)
Important notice:
To use this software version it is necessary to have a central control unit IMAG-CG with a S/N from IKEC0342B on or alternatively an updated version with EEPROM version 3.x
Instruments with S/N IKEC0342 or higher are also bundled with their LED arrays (sticker on units). Central control units and imaging heads MUST NOT be interchanged with other systems.
For finding out how your system can be updated, please inquire under info@walz.com.
PC software PhytoWin V2.13
Please read the installation note carefully before software installation!
PC software PhytoWin V3.t99q
not suitable for PHYTO-PAM (Predecessor Model)
Windows XP SP2 requires PhytoWin software version V2.10 and PHYTO-PAM instrument (EPROM) software version 010106. EPROM version can be checked with
Software update KLAS-100 V3.016
Version 3.016 is an update and should be installed on top of the existing software. The update leaves the DMP files, PAR lists, experimental data, etc. untouched. It is of course always a good idea to make a backup of the KLAS-100 folder, before installing the update.
Sample data for PC software WinControl-3. To view sample data, install WinControl-3, unzip data and double-click on file ICandLCsample.pam
How to view sample data
Sample data for PC software PamWin
How to view sample data
Sample data for PC software PamWin
Sample data for PC software WinControl-3
Install WinControl-3 on Windows PC, download sample data, to view sample data, double-click on file.
Instruction manual for PAR measurements of thin fiberoptics
Instruction manual for 3010-GWK1
Download from Walz website the for the DIVING-PAM-II battery. Print entire protocol (6 pages) and enclose it in transport case
Some DIVING-PAM-II fluorometers (up to S/N UWFD0110) contain a lithium manganese oxide battery requiring the following safety measures for shipping:
Discharge battery to 30%. Use original DIVING-PAM-II/T transport case for shipping
How to view sample data
Sample data for PC software PamWin
Instruction manual for ULM-500
Instruction manual for US-SQS/L
Paper "Chlorophyll Fluorescence: New Instruments for Special Applications" (Dr. Schreiber)
Instruction manual for LED-Panel RGBW-L084
Walz PAM Tutorial: Chlorophyll Fluorescence and Photosynthesis: Simple Experiments with the JUNIOR-PAM Chlorophyll Fluorometer.
Instruction manual for KF-18/2B and KF-24/6B
Instruction manual for HEXAGON-IMAGING-PAM
Manual for NADPH and Acridine Orange Fluorescence Emitter-Detector Modules
Manual for DUAL-K25 Quartz Glass Cuvette for Suspensions
Manual for 3010-DUAL Dual-PAM Gas-Exchange Cuvette
Manual on MODULAR and FIBER versions of DUAL-PAM systems, and thorough description of the DualPAM software.
Estimating epidermal UV-A and UV-B screening in leaves with a XE-PAM Fluorometer.
By Erhard PfΓΌndel.
This communication recapitulates step-by-step how epidermal transmittance for ultraviolet (UV) radiation can be determined by chlorophyll fluorescence measurements using a XE-PAM fluorometer.
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PAN07006.pdf
High-end chlorophyll fluorescence analysis with the MULTI-COLOR-PAM.
I. Various light qualities and their applications.
By Ulrich Schreiber, Christof Klughammer and Jörg Kolbowski.
The paper provides an overview on spectral properties of pulse-modulated measuring light (ML) and actinic light of the MULTI-COLOR-PAM, and outlines applications of these various light sources. The ML can be applied from 10 to 200000 Hz, thus enabling continuous monitoring of Fo fluorescence as well as measurements of fast induction kinetics in the sub-ms time range including O-I1 fluorescence rise kinetics to determine optical cross-section of PS II, Sigma(II)λ. Based on Sigma(II)λ, PAR, in units of μmol quanta/(m2 s), can be converted into PAR(II), in units of PS II effective quanta/s. A fluorescence-based electron transport rate ETR(II) = PAR(II) x Y(II)/Y(II)max is defined, which can describe the rate of electron transport even in dilute suspensions of unicellular algae and cyanobacteria.
Relevant PAN E-Journal for MULTI-COLOR-PAM
PAN11001.pdf
Photosynthesis in ripe strawberries (Fragaria x ananassa) recording by a Maxi-Imaging-PAM.
By Oliver Meyerhoff and Erhard PfΓΌndel.
The presence of functioning PS II in fruits (achenes) of strawberries is demonstrated using a Maxi-Imaging-PAM instrument.
PAN07004.pdf
New accessory for the Dual-PAM-100: The P515/535 module and examples of its application.
By Ulrich Schreiber and Christof Klughammer.
The technical features of the P515/535 module are outlined and some typical examples of application are presented.The device provides information on membrane potential, membrane energization ("scattering"), and proton gradient, as well as on proton and electron fluxes.
PAN07001_ed2.pdf
Saturation Pulse method for assessment of energy conversion in PS I.
By Christof Klughammer and Ulrich Schreiber.
The paper summarizes the theoretical background of the saturating pulse method which is utilized by the DUAL-PAM-100 instrument to determine the quantum yields of photochemical energy conversion and nonphotochemical energy dissipation in PS I.
PAN07002.pdf
Non-photochemical fluorescence quenching and quantum yields in PS II and PS I: Analysis of heat-induced limitations using Maxi-Imaging-PAM and Dual-PAM-100.
By Ulrich Schreiber and Christof Klughammer.
In this article the large potential of combined measurements with the Maxi-version of the Imaging-PAM and the Dual-PAM-100 are demonstrated.
PAN07003.pdf
Monitoring the effects of reduced PS II antenna size on quantum yields of photosystems I and II using the Dual-PAM-100 measuring system.
By Erhard PfΓΌndel, Christof Klughammer and Ulrich Schreiber.
The Dual-PAM-100 is employed to analyze Chl b-less barley leaves (Hordeum vulgare cv. Donaria mutant chlorina-f2 2800) and the corresponding wild-type leaves. The results show that the small PS II antenna size in the mutant affects both, PS I and PS II photochemistry.
PAN07005.pdf
Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the Saturation Pulse method.
By Christof Klughammer and Ulrich Schreiber.
The fate of excitation energy in PS II is comprehensively described by the complementary quantum yields Y(II) + Y(NPQ) + Y(NO) = 1. It is shown that the simple expressions for Y(NO) and Y(NPQ) proposed by Genty et al. (1996), which do not contain Fo', are fully equivalent to the much more complex expressions of Kramer et al. (2004) and are valid for both lake and puddle models. The practical meaning of the complementary quantum yields is discussed.
PAN078007.pdf
New NADPH/9-AA module for the DUAL-PAM-100: Description, operation and examples of application.
By Ulrich Schreiber and Christof Klughammer.
The NADPH/9-AA module is a new accessory of the DUAL-PAM-100 system which excites fluorescence at 365 nm and detects fluorescence in the 420 to 580 nm range. The new module permits measuring of light-induced changes of NADPH fluorescence in suspensions of isolated chloroplasts, algae and cyanobacteria. Technical features of the module are outlined and examples of application are introduced. Also simultaneous measurements of chlorophyll (Chl) and NADPH fluorescence are presented. It is show that saturation pulses can serve for estimating the extent of NADP reduction in the steady state.
Β pan09001_rev04.pdfΒ
Relevant PAN E-Journals for DUAL-PAM-100
General Terms and Conditions for Products and Services of Heinz Walz GmbH
Manual for Mini-Quantum/Temp.-Sensor 2060-M and 2065-M, Arabidopsis Leaf Clip 2060-B, Fiberoptics Holder for Surfaces 2060-A
For 1st generation instruments DIVING-PAM, MINI-PAM, MICROSCOPY-PAM and MICROFIBER-PAM
List of abbreviation used by WinControl V2.08 to describe instrument settings.
For 1st generation instruments DIVING-PAM, MINI-PAM, MICROSCOPY-PAM and MICROFIBER-PAM.
Instruction manual for WinControl V2.08
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For 1st generation instruments DIVING-PAM, MINI-PAM, MICROSCOPY-PAM and MICROFIBER-PAM
PC software WinControl V2.08Β
PAM-CONTROL requires instrument (EPROM) software version 2.00 or higher!
PC software PamWin V2.00f for predecessor model PAM200/2100
PAM-2000 requires instrument (EPROM) software version 1.56!
VIEW mode without prior MEASURE mode requires copying files into C:\PamWin\Data
PC software PamWin V2.00f for predecessor model PAM200/2100
PAM-2000 requires instrument (EPROM) software version 1.56!
VIEW mode without prior MEASURE mode requires copying files into C:\PamWin\Data