COMPACT Version

PHYTO-PAM-II

In the COMPACT version of the PHYTO-PAM-II all components including the measuring chamber are contained in a single compact housing. Hence, except for the USB-cable for communication with the PC, normal operation does not involve connection of external cables, so that this device is particularly suited for on-deck and field applications. It features five wavelengths of fluorescence excitation and actinic illumination (440, 480, 540, 590 and 625 nm). In addition, also white light for actinic illumination and far-red light for preferential excitation of photosystem I is provided.

Next to the fluorescence based estimation of chlorophyll content the PHYTO-PAM-II offers PAM saturation pulse analysis of chlorophyll fluorescence to determine the photosynthetic performance of different algal groups. Also a fast kinetic mode for the determination of the wavelength-dependent absorption cross section of PS II, σII(λ) is provided.

The compact unit can be equipped with the Flow-Through Cuvette PHYTO-II/FT. Together with the 0-10 V Universal Pump Control PHYTO-II/FT/I common laboratory peristaltic pumps can be used for software controlled automated sampling.

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The COMPACT version of the PHYTO-PAM-II features a 15 mm (outer Φ) round cuvette, which is illuminated from the bottom, with fluorescence being detected at right angle. In this way, the whole sample is illuminated homogeneously and a representative part of the sample is monitored.

Reference spectra measured with dilute suspensions of pure cultures, corresponding to 5-point fluorescence excitation spectra, serve for automated online deconvolution of up to 4 different pigment types of phytoplankton (green algae, diatoms/dinoflagellates, cyanobacteria and phycoerythrin containing organisms, like cryptophytes).