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LSA-2050
LEAF-STATE-ANALYZER
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The LEAF-STATE-ANALYZER LSA-2050 is a handheld device for non-invasive leaf analysis. The device analyses three areas that are related to the state of health of the plant: (1) the extent of protection from ultraviolet and strong visible radiation, (2) the chlorophyll concentration, and (3) the maximum photochemical quantum yield of photosystem II, FV/FM. In summary, the LEAF-STATE-ANALYZER LSA-2050 provides a picture of stress effects and a plant's ability to cope with stress.
Outstanding Properties of the LEAF-STATE-ANALYZER
- Measures protection against the particularly harmful UV-B radiation
- Probes screening at four wavebands: UV-B, UV-A, blue, and green
- Provides the stress factors chlorophyll content and photosystem II damage
Probing a leaf after predarkening in a Darkening Bag (LSA-2050/DB). Dark acclimation ...
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Functional units of the LEAF-STATE-ANALYZER LSA-2050. "Emitter Detector Head": LEDs ...
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Battery compartment and USB-C port for data transfer.
LEAF-STATE-ANALYZER LSA-2050 with lower part removed for fruit measurements.
General Features LEAF-STATE-ANALYZER
The LEAF-STATE-ANALYZER LSA-2050 measures radiation screening by the efficiency of fluorescence excitation. The four different excitation wavebands used can be assigned to four pigment groups: UV-B and UV-A to hydroxycinnamic acids and flavonoids, respectively [1], blue to carotenoids [2], and green to anthocyanins [3]. Absorbance values indicating relative flavonoid and anthocyanin concentration are provided.
Chlorophyll concentration is measured by the Cerovic method [4]. The method excels by high response even at high chlorophyll concentrations. Photosystem II is analyzed by the well-proven PAM fluorescence/saturation pulse method [5]. With each measurement, GPS data, leaf orientation, and the direction of sun radiation are logged.
[1] Bilger W, Veit M, Schreiber L, Schreiber U (1997) Measurement of leaf epidermal transmission of UV radiation by chlorophyll fluorescence. Physiol Plant 101: 754–763
[2] Nichelmann L, Schulze M, Herppich WB, Bilger W (2016) A simple indicator for non-destructive estimation of the violaxanthin cycle pigment content in leaves. Photosynth Res 128: 183-193
[3] Cerovic ZG, Moise N, Agati G, Latouche G, Ben Ghozlen N, Meyer S (2008) New portable optical sensors for the assessment of winegrape phenolic maturity based on berry fluorescence. J Food Compos Anal 21: 650-654
[4] Cerovic ZG, Masdoumier G, Ben Ghozlen N, Latouche G (2012) A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids. Physiol Plant 146: 251–260
[5] Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51-62