Specifications for JUNIOR-PAM

Optoelectronic Unit JUNIOR-PAM/W (WHITE Version)

Modulated fluorescence excitation: White LED (wavelengths of maximum emission: 445 and 545 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 650 nm is measured

Actinic light: Same LED as used for modulated light. PAR at 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide: between 25 and 1500 μmol m-2 s-1. Maximum PAR of saturation pulses, 7000 µmol m-2 s-1

Optoelectronic Unit JUNIOR-PAM and JUNIOR-PAM/W

Housing: Aluminum housing with USB type B socket, M8 4-pole socket to connect the JUNIOR-PAM monitoring leaf clip (accessory), port for JUNIOR-PAM light guide, and swivel-mounted sample support

Far-red light: LED with 745 nm maximum emission wavelength for selective excitation of photosystem I

Fluorescence detection: PIN photodiode protected by long-pass filter. Selective window amplifier to measure pulse-amplitude modulated (PAM) fluorescence

Communication: Standard USB communication via 1.2 m USB-cable type A-B

Power supply: 5 V DC supplied by the USB-cable type A-B used for communication

Power consumption: 100 mW at normal operation and 500 mW during saturation pulse

Dimensions: 11.5 cm x 6.5 cm x 3 cm (L x W x H)

Weight: 200 g

Operating temperature: 0 to + 40 °C

Operating humidity range: 35 to 85% RH (to avoid condensation)

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. PAR and leaf temperature when Monitoring Leaf-Clip JUNIOR-BD is connected. Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmability: Fully programmable using batch file language

Computer Requirements: 1 free USB socket. Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0

Miscellaneous items included

Light guide: 50 cm x 1.5 mm (length x diameter) plastic fiber

Sample clips: Open leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 60°) and magnetic leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 90°)

Fluorescence standard: 3 cm x 2 cm fluorescence foil

Polishing set: Set for fiber tips including various polishing pads

USB cable: A-B USB cable

Software on USB memory key: Including latest WinControl-3 software

Transport Case JUNIOR-T: Grey plastic box with handle. Interior: convoluted foam padding. Dimensions: 29 cm x 25 cm x 6 cm (L x W x H). Weight: 510 g

Monitoring Leaf-Clip JUNIOR-BD

Micro quantum sensor: Selective PAR measurement, 0 to 2500 μmol m-2 s-1 PAR

Thermocouple: Ni-CrNi, -20 to +60 °C

Leaf temperature: -20 to +60 °C

Power supply: JUNIOR-PAM special socket

Cable length: 100 cm

Dimensions: 13 cm x 2.5 cm (max.) x 5.5 cm (max.) (L x W x H)

Weight: 162 g (including cable)

Extra-long Light-Guide

100 cm x 1.5 mm (length x diameter) plastic fiber for difficult accessible samples or underwater measurements