Specifications for MULTI-COLOR-PAM-II

Power-and-Control-Unit MCP-II-C

General design: ARM microcontroller (480 MHz), Fast Kinetics up to 128,000 points with 14 bit resolution, ST Kinetics up to 15,000 points with 14 bit resolution, unlimited storage for Slow Kinetics

Sockets: 4 sockets for measuring light and actinic light of MCP-II-E Multi-Color Emitter Head and measuring light/auxiliary detector and ST supply MCP-II-EDST, 2 sockets for signal detection by MCP-II-D1 and MCP-II-D2DST Detector Heads, charge socket or Battery Charger MINI-PAM/L, output socket for PHYTO-MS Miniature Magnetic Stirrer, 4 BNC sockets for 5 V trigger in and trigger out signals and ST trigger in and trigger out signals, input socket for US-SQS/WB Spherical Micro Quantum Sensor or US-MQS/WB Mini Quantum Sensor, input socket for auxiliary devices, USB socket

Communication: USB 2.0, USB 3 compatible

User interface: Windows computer with PamWin-4 software

Power supply: standard: no sealed lead-acid battery; Battery Charger MINI-PAM/L (100 to 240 V AC)

Dimensions: 31 cm x 16 cm x 33.5 cm (W x H x D)

Power consumption: 6 W

Weight: 3.64 kg

Operating temperature: -5 to +40 °C

Multi-Color Emitter Head MCP-II-E

Chip-on-board multi-wavelength measuring light LED emitter: 400, 440, 480, 540, 590, 625 nm for pulse-modulated measuring light; 20 intensity settings and 14 14 pulse frequency settings

Chip-on-board multi-wavelength actinic LED array: 440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, max. 5000 µmol photons m-2 s-1; saturating single turnover flashes, max. 200000 µmol photons m-2 s-1; multiple turnover pulses, max. 12000 µmol m-2 s-1 PAR, adjustable between 1 and 800 ms

Far-Red LED: peak wavelength 730 nm

Dimensions: 10.5 cm x 5.5 cm x 7 cm (L x W x H)

Weight: 500 g (incl. cables, 1 m long)

Multi-Color Detector Head MCP-II-D1

Signal detection: PIN photodiode with special pulse preamplifier for measuring fluorescence changes with maximum time resolution of 10 µs

Filter box: for up to 14 mm filter thickness

Standard detector filter: long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710

Dimensions: 6.9 cm x 9.8 cm x 6.4 cm (L x W x H)

Weight: 355 g (incl. cables, 1 m long)

Emitter Head for fast Fluorescence Measurements MCP-II-EDST

Signal generation and detection: High quality 450 nm ST-flashes of variable duration and intensity up to 1 mol photons m-2 s-1; Actinic light (450 nm); Far Red light (740 nm); STK-detector for surface detection of direct fluorescence with sub-µs time resolution

Dimensions: 15.4 cm x 5.2 cm x 7 cm

Weight: 570 g (incl. cables, 1.2 m long)

Combined Detector Head for Double Channel and Fast Fluorescence MCP-II-D2DST

STK & PAM-Detector (switchable): includes: PAM-detector equivalent to MCP-II-D1 and STK-detector for non-modulated fluorescence with sub-µs time resolution

Filter box: for up to 14 mm filter thickness

Standard detector filter: long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710

Dimensions: 6.9 cm x 9.8 cm x 6.4 cm (L x W x H)

Weight: 550 g (incl. cables, 1.2 m long)

Power Supply MINI-PAM/L

Input: 90 to 264 V AC, 47 to 63 Hz

Output: 19 V DC, 3.7 A

Operating temperature: 0 to 40 °C

Dimensions: 15 cm x 6 cm x 3 cm (L x W x H)

Weight: 300 g

It should be noted that the MULTI-COLOR-PAM-II does not contain a battery anymore.

Optical Unit for Suspensions ED-101US/MD

Design: Black-anodized aluminum body with central 10 x 10 mm standard glass cuvette; ports for attachment of the Emitter/Detector Heads MCP-II-E, MCP-II-D1, MCP-II-EDST and MCP-II-D2DST, and Miniature Magnetic Stirrer PHYTO-MS

Weight: 750 g

Stand ST-101

Stand for mounting the Optical Unit ED-101US/MD (suspensions) or the Linear Positioning System 3010-DUAL/B (leaves) or MCP-II-BK (leaves)

Transport Box PHYTO-T

Design: Aluminum box with custom foam packing for MULTI-COLOR-PAM-II and accessories

Dimensions: 60 cm x 40 cm x 34 cm (L x W x H)

Weight: 5 kg

System Control and Data Acquisition

Software: PamWin-4 System Control and Data Acquisition Program for operation of the instrument via PC or laptop, data acquisition and analysis

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Time-resolved Saturation Pulse: Fast polyphasic rise and decay kinetics (time resolution up to 10 µs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB

Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 not constituting PS II chlorophyll fluorescence)

Fitting Routines

Fitting routine for the fast fluorescence O-I1 to determine the functional absorption cross-section of the PS II antennae (Sigma) needed for the determination of PS II-specific electron transport rates.

Fitting routine for exponential decay (e.g. QA– reoxidation after a single turnover flash) or rise of a signal with up to three exponentials.

Choice of two fitting routines for light curves (determination of cardinal points α, Ik and ETRmax).

Computer Requirements

Operating system: Microsoft Windows 10 (32 and 64 bits) and 11; computers that can run these operating systems, can also work with PamWin-4.