MINI-PAM-II
Version:
Photosynthesis Yield Analyzer
MINI-PAM-II Photosynthesis Yield Analyzer and leaf clip 2035-B.
MINI-PAM-II
Sensitivity, small dimensions, reliability under rugged conditions, and simple execution of fluorescence analysis make the MINI-PAM-II the standard for PAM fluorometry in field research. The MINI-PAM-II combines well-tested fiberoptics with the most modern LED and computer technology. A sun-readable touchscreen serves as easy-to-use interface for field studies. The fluorometer automatically calculates and displays all relevant parameters of saturation pulse analysis.
The new leaf clip MINI-PAM-II/POROMETER complements the analysis with precise information on water evaporation, stomatal conductance and other parameters important for understanding stomatal function and photosynthetic performance.
Accessories for MINI-PAM-II: MINI-PAM-II/POROMETER
The MINI-PAM-II/POROMETER is a new leaf-clip for the MINI-PAM-II for combined measurements of stomatal conductance and chlorophyll a fluorescence. It measures water evaporation and stomatal conductance of plant leaves and needles to determine stomatal function, which is strongly influenced by stress factors acting on the plant.
This new device is a powerful tool for rapid screenings, high-throughput stress evaluations, or detailed plant analysis with well-established protocols like induction-curves or light-curves. Being lightweight and compact, the device is ideally suited for research from the field to the laboratories. With the built in GPS, accelerometer, gyroscope, and magneto scope sensor the porometer adds geospatial information including angle of incidence of sun radiation and the leaf angle.
The porometer delivers its data every second. The typical length of a measurement will vary depending on conditions such as flow rate and sample. At a standard flow rate of 100 µmol s-1 and an expected stomatal conductance of 100 mmol m-2 s-1 the average measurement duration is less than 15 seconds. The included automatic stability determination is particularly useful for single field measurements. Simply press the button, wait for the data to meet the stability criteria, and the measurement is automatically executed. An acoustic signal can be included, if desired.
General Features MINI-PAM-II
Energy-efficient LED sources, storage capacity for 27,000 data sets, and easy replaceable off-the-shelf batteries permit extensive measurements in remote places.
The far-red light (735 nm LED) of the MINI-PAM-II preferably excites photosystem I. Low intensity far-red light induces nearly no charge separations in photosystem II. A special measuring routine uses this far-red light to determine the F0’ fluorescence level, which is important to correctly assess the redox state of photosystem II reaction centers.
Expandable through accessories such as various leaf clips, external multi-color lamp, miniature spectrometer, suspension cuvette, optical oxygen sensor, and barcode scanner.
In experiments using the internal actinic light source, the light intensity at sample level can be monitored online using an internal light sensor. This internal sensor must be calibrated against an external light sensor.
USING THE MINI-PAM-II FOR SUSPENSION MEASUREMENTS
The MINI-PAM-II can also be used in combination with a suspension cuvette (KS-2500) and a stirring unit (MKS-2500). The cuvette can hold 400 µl of suspension. The photo shows a configuration allowing the simultaneous measurement of fluorescence (fiber stabilized by a DUAL-BA) and oxygen with a FirestingO2 optode (Pyroscience). In the absence of the optode, for example, inhibitors can be added to the sample with a syringe along this route. A water bath can be connected to the suspension cuvette to control the temperature of the sample.
