MICRO-PAM

Version:

Product

Compact Fluorometer for Long-term Monitoring of Photosynthesis

MICRO-PAM

Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

General design
Housing
Polymer housing with optical block and fiber connector on one side, and a combined power line/RS485 socket on the opposite side
MICRO-HEAD/BK Leaf Clip
Consisting of 2 aluminum frames (3.5 x 2.5 cm), held together by magnet, 0.4 cm distance between sample plane of leaf clip and lightguide, angle between sample plane and optical light guide: 60°
Cables
RS 485 data/power cable, 10 m standard length, connecting MICRO-HEAD/3B and MICRO IB4 PC Interface Box. RS 485 adapter cable, 0.6 m long, to connect MICRO-HEAD/3B to optional PC Interface Box MONI IB4/LAN or to optional MONI DA system for data acquisition
Flexible stand
Baseplate of acrylic glass 10 x 10 x 0.5 cm (L x W x H); flexible goose neck consisting of 20 links, 1.7 cm maximum diameter, 30 cm length, one end with metal thread equipped with 1 butterfly nut, 1 nut and 2 washers. Weight 114 g
Dimensions
Complete head with leaf clip, 13.5 x 3.5 x 4 cm (L x W x H)
Power consumption
Peak loads during saturating pulses 3 W. During measuring mode 0.15 W
Operating temperature
-15 to +40 °C
Weight
96 g
Light Emission
Modulated fluorescence excitation
Blue power LED (typical peak wavelength 460 nm, full width at half maximum 22 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequencies (100 Hz)
Actinic light
Same power LED as for modulated light. At sample level of leaf clip, 3000 μmol m-2 s-1 maximum PAR of actinic light, 8000 μmol m-2 s-1 maximum PAR of saturating flashes
Sensors
Fluorescence
PIN-photodiode protected by longpass filter (50% transmittance at 645 nm). Selective window amplifier to measure pulse amplitude modulated (PAM) fluorescence.
Photosynthetically active radiation (PAR)
External LS-C sensor for selective PAR measurement, range 0 to 7000 μmol m-2 s-1, cosine-corrected for light incident at angles between -30° to +30° from the surface normal
Temperature
Thermocouple: Ni-CrNi, wire diameter 0.1 mm, -20 to +60 °C
Humidity
Capacitive-type humidity sensor on a specialized analog and digital integrated circuit, 0 - 100% relative humidity
General design
Modulated fluorescence excitation
Amber power LED (typical peak wavelength 598 nm, full width at half maximum 27 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequencies (100 Hz)
Actinic light
Same power LED as for modulated light. At sample level of leaf clip, 3000 μmol m-2 s-1 maximum PAR of actinic light, 8000 μmol m-2 s-1 maximum PAR of saturating flashes
Further specifications
Same as Measuring Head MICRO-HEAD/3B
Housing
Aluminum case with RS-232, USB-B, Ethernet, power supply sockets, and four M12 5-pole sockets for RS-485 communication
Interfacing
The interface box connects a computer with up to four MICRO-HEAD/3B (or one MONI-DA). RS-485 serial data communication is used between interface box and MICRO-HEAD/3B or MONI-DA. RS232, USB or Ethernet communication is used between interface box and computer
Recommended maximum cable lengths
To computer via USB and RS-232: 2 m. To computer via Ethernet, 100 m. To MICRO-HEAD/3B via RS-485: 10 m. To MONI-DA via RS-485, 100 m
Dimensions
12 x 9.3 x 3 cm (L x W x H)
Weight
400 g
Operating temperature
0 to +40 °C
Power supply
Input: 100 to 240 V AC, 50 to 60 Hz. Output: 19 V DC, 3.7 A. Dimensions: 13.2 x 5.8 x 3 cm (L x W x H). Weight: 310 g
Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10/11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Measured parameters
Ft, F0, FM, F, F0' (also calculated), FM'. Calculated: F0' (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate). Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves
Further date acquired
PAR, leaf temperature, humidity
Additional feature
Automatic determination of signal offset for all light intensities and all gain levels
Communication Protocol
USB
Computer Requirements
Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0
Housing
Robust water-proof cylinder consisting of a polyvinyl chloride (PVC) tube and poly-oxymethylene (POM) endplates. One endplate with 2 male M12 5-pole sockets connected in parallel (MONI-IB4/LAN communication, charging voltage), one male M12 5-pole socket for auxiliaries, and 7 female M12 5-pole sockets (MICRO-PAM measuring head communication)
Dimensions
Cylinder with diameter of 16 cm and length of 24 cm
Data management
Dual data storage on internal 8 MByte circular flash buffer and an industrial grade 512 MByte removable microSD flash card. Wireless data transfer via cellular phone or satellite modem. Online data transfer using RS-485 serial data communication.
Power consumption
5 mW in standby mode. Operating mode, depends on the number of MICRO-PAM measuring heads connected (see MICRO-HEAD/3B power consumption)
Battery
12 V / 7.5 Ah (96 Wh) LiFePO4 battery.
Operating temperature
-30 to +60 °C
Weight
5.4 kg
Housing
Aluminum case with USB-B socket, power supply socket, and M12 5-pole socket for RS-485 communication
Links
The interface box connects a computer with a MONI-DA using RS-485 serial data communication. The same line is used to charge the MONI-DA battery. USB communication is used between interface box and computer
Recommended maximum cable lengths
To computer via USB: 2 m. To MONI-DA via RS-485,
100 m
Dimensions
9.7 x 6.3 x 3.5 cm (L x W x H)
Housing
Aluminum case with USB-B socket, power supply socket, and M12 5-pole socket for RS-485 communication
Weight
270 g
Recommended maximum cable lengths
To computer via USB: 2 m. To MONI-DA via RS-485,
100 m
Operating temperature
0 to +40 °C
Design
Polycrystalline silicon panel. Highly resistant to water, abrasion, hail impact and other severe weather conditions. Equipped with a 4 m cable and plug for the AUX or INPUT socket of the Data Acquisition System MONI-DA. Several panels can be connected in parallel to provide sufficient power under conditions of low insolation
Electrical characteristics
Vmax, 15.0 V, Imax, 0.63 A (at 1000 W/m2 sunlight). VOC, 19.6 V. ISC, 0.80 A
Dimensions
50 x 35 cm (L x W)
Design
Polycrystalline silicon panel. Highly resistant to water, abrasion, hail impact and other severe weather conditions. Equipped with a 4 m cable and plug for the AUX or INPUT socket of the Data Acquisition System MONI-DA. Several panels can be connected in parallel to provide sufficient power under conditions of low insolation
Weight
1.2 kg (incl. cable and plug)
Electrical characteristics
Vmax, 15.0 V, Imax, 0.63 A (at 1000 W/m2 sunlight). VOC, 19.6 V. ISC, 0.80 A
Design

Aluminum box with custom foam packing MICRO-PAM

Dimensions

60 cm x 40 cm x 34 cm (L x W x H);
60 liter

Weight

4.9 kg

Design
Aluminum box with custom foam packing for PAM-CONTROL and accessories
Dimensions
60 cm x 40 cm x 25 cm (L x W x H)
Weight

4,7 kg

Accessories

Design
Two double-ball arm segments, two ball end pieces with photo thread (1/4” external), to mount a measuring head and a base plate with photo thread (1/4" internal), respectively.
Dimensions
39 cm (L max.)
Weight
410 g
Housing
Aluminum case with one M12 5-pole connector and four M8 5-pole connectors
Links
The interface box connects up to four MICRO-PAM measuring heads to one single MONI-BUS port of a MONI-DA data acquisition system
Recommended maximum cable lengths
10 m between MICRO-PAM measuring head and MICRO-HUB, and between MICRO-HUB and MONI-DA
Dimensions
10 x 6 x 3.5 cm (L x W x H)
Weight
228 g
Operating temperature
-30 to +40 °C
Recommended maximum cable lengths
10 m between MICRO-HEAD/3B and MICRO-HUB, and between MICRO-HUB and MONI-DA
Design
Weatherproof cylinder made of POM (Polyoxymethylene) containing a standard WiFi modul. One endplate made of POM, the other endplate made of Plexiglas with 5-pole M12 plug connector. Includes a 10 m cable to connect the modem to the AUX port of the MONI-DA. Data transfer requires connection to a compatible WiFi network or hotspot (2.4 GHz, IEEE 802.11 b/g/n, WPA2-PSK)
Dimensions
19.5 cm (L) 3.27 cm (Ø)
Weight
140 g (modem), 320 g (cable)

Specification depend on available electronic components at the time of order