DIVING-PAM-II

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Product

Underwater Fluorometer with Miniature Spectrometer

DIVING-PAM-II

Design
POM tube, at one end face, optical sensor spot fixed by a Perspex disk with central hole and PT100 resistance thermometer, at the other end face, 4-pole underwater socket. Temperature-compensated oxygen measurement by a high precision optical oxygen meter connected to the sensor spot by fiberoptics. Including a 2 m underwater cable 000130204945, two spare oxygen sensor spots OXSP5, and a holder (weight 75 g, maximum dimensions 6.5 cm x 6 cm x 12 cm, L x W x H) to attach both the DIVING-PAM-II/O2 and the spectrometer MINI-SPEC to the DIVING-PAM-II optoelectronic unit, consisting of the mounting brackets 000246001714 and 000246003914 and 1 ring holder 000244905514.
Maximum diving depth
50 m
Dimensions
3.25 cm diameter, 17.5 cm length
Weight
135 g
Dimensions
25 m length, 6 mm diameter
Weight
1.25 kg
Dimensions
50 m length, 6 mm diameter
Weight
2.5 kg
Including charger DIVING-II/L15
Input: 100 V to 240 V AC, 50 to 60 Hz. Output: 15 V DC, 4.65 A. Operating temperature: -5 to +45 °C, (non-condensing). Dimensions: 13 cm x 5.5 cm x 3 cm (L x W x H). Weight: 350 g including cables
Measuring light
Blue LED (470 nm ± 8 nm tolerance), standard modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz and 100 Hz, measuring light PAR at standard settings = 0.05 μmol m-2 s-1. Fluorescence at wavelengths greater than 630 nm is measured
Actinic light
Same blue LED as for measuring light, maximum actinic PAR = 3000 μmol m-2 s-1, maximum PAR of saturation pulses = 6000 μmol m-2 s-1 adjustable at increments of 500 μmol m-2 s-1
Measuring light
Red LED (655 nm ± 5 nm tolerance), modulation frequencies and PAR as described for DIVING-PAM-II/B. Fluorescence at wavelengths greater than 700 nm is measured
Actinic light
Same red LED as for measuring light, maximum PAR of actinic light and saturation pulses as described for DIVING-PAM-II/B
Fluorescence detection
PIN photodiode protected by long-pass and a short-pass filters, 12 bit signal resolution
Pressure and temperature
Piezo-resistive pressure sensor and temperature sensor. Pressure is converted in meters of diving depth, range 0 to -50 m, displayed at 0.1 m intervals. Temperature, range -10 °C to +60 °C, displayed at 0.1 °C intervals. Sensor module with gel protection and antimagnetic stainless steel cap
Data storage
Flash memory, 8 MB, providing memory for more than 27,000 saturation pulse analyses
Display
Backlit 160 x 104 dots (78 x 61 mm) transflective B/W screen
Control elements
10 infrared reflection switches, pushbutton for saturation pulses, pushbutton to switch device on/off and to lock/unlock reflection switches
WLAN
Wireless LAN Interface (2.4 GHz, IEEE 802.11 b/g/n, WPA2-PSK) Access Point Mode
Design
Plexiglas tube with Plexiglas end plates, one with waterproof fiberoptics port
Mountings
2 anodized aluminum rods (diameter 15 mm) mounted parallel to the fluorometer body. Anodized aluminum holder for fiber optics. Connections for carrying belt and miniature spectrometer MINI-SPEC
Ports
AUX1 and AUX2, 4-pole, for miniature spectrometer MINI-SPEC; for laboratory use via special adapter: Fiber-Optic Oxygen Meter FireStingO2 or Leaf Clip Holder 2035-B. OUT1 and OUT2, 6-pole, input for trigger signal from Universal Sample Holder DIVING-II-USH; for laboratory use via special adapter: External LED Light Source 2054-L. INPUT, 6-pole, for RS-485 communication and charging of internal battery
Battery
Lead acid battery 8.0 V / 3.5 Ah (28 Wh) providing power for more than 1300 yield measurements
Maximum diving depth
50 m
Operating temperature
-5 to +45 °C
Dimensions
Diameter 19 cm, length 39 cm
Weight
3.9 kg
Design
POM tube, at one side, port for light detection, port for fluorescence excitation by blue (452 nm max) or green (525 nm max) LEDs, and port for white light from a tungsten lamp for reflection measurements; at the opposite side, 4-pole underwater socket
Spectrometer module
Hamamatsu micro-spectrometer, spectral range: 400 to 800 nm, spectral resolution: between 8 and 10 nm. Maximum PAR: 4000 µmol m-2s-1 for illumination having spectral characteristics similar to sunlight
Dimensions
3.25 cm diameter, 17.5 cm length max
Weight
135 g
Flat Entrance Optics SPEC/P

Design: Hard-anodized aluminum rod of 10 mm diameter and 50 mm length, at one end with lateral light entrance through a 5 mm diameter diffusor and the opposite end inserted in a mounting plate (diameter 33 mm, height 5 mm). Aluminum rod with internal light guide

Fluorescence and Reflection Optics SPEC/R
Design: Spectrometer cap consisting of POM: maximum diameter 35 mm, height 13 mm, weight 16 g. With central 5 mm x 16 mm groove accommodating Perspex light guides for blue and green for fluorescence excitation, and for white light for reflection measurements. With 3 mm diameter central drilled hole as light channel to the detector window of the spectrometer. Including sample cap to fix sample (maximum diameter 40 mm, height 10 mm, weight 8 g). Spectrometer and sample caps padded with foam rubber, both parts have magnets build-in to attract each other and, thus, hold the sample
PAR Calibration Block 000160101439
Design: POM block with drill hole for entrance optics of the Miniature Spectrometer. Oriented at an angle of 60° and 90° relative to the spectrometer port are drill holes for the Fiber Optics DIVING-F
Dimensions
4.15 cm x 2 cm x 5 cm (L x W x H)
Weight
40 g
Design
Randomized 70 µm glass fibers forming single plastic shielded bundle with stainless steel adapter ends
Dimensions
Active diameter 5.5 mm, outer diameter 8 mm, length 150 cm
Weight
340 g
Input

100 V to 240 V AC, 50 to 60 Hz

Output
12 V DC, 5.5 A
Operating temperature
-5 to +45 °C, (non-condensing)
Weight
350 g including cables
Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

Housing
Aluminum case with USB-B port, socket for power supply MINI-PAM-II/N, and waterproof 6-pole socket for RS-485 communication
Function
Connects computer and DIVING-PAM-II. RS-485 serial data communication is used between box and DIVING-PAM-II, USB communication is employed between interface box and computer. Recommended maximum cable lengths: 50 m RS-485 cable, 2 m USB cable. Standard USB-A to USB-B cable included
Dimensions
9.7 cm x 6.3 cm x 3.5 cm (L x W x H)
Weight
270 g
Operating temperature
-5 °C to + 40 °C
Length
5 m
Weight
500 g
Design
Metal clip with fiber holder and 11 mm diameter sample hole: 5.5 cm x 1.4 cm (L x W)
Fiber holder
1.2 cm length, mounted 0.7 cm above base, with lateral screw to fix fiber optics. Angle between fiber optics axis and sample plane: 60°. Two spacer rings to vary the distance between fiber end and leaf surface
Design
Rugged, hard plastic outdoor case with wheels, pull-out handle and custom foam packing
Dimensions
57 cm x 47 cm x 27 cm (L x W x H)
Weight
7.7 kg

Some DIVING-PAM-II fluorometers (up to S/N UWFD0110) contain a lithium manganese oxide battery requiring the following safety measures for shipping:

Discharge battery to 30%. Use original DIVING-PAM-II/T transport case for shipping.

Download from Walz website the testing protocol for the DIVING-PAM-II battery. Print entire protocol (6 pages) and enclose it in transport case.

Visibly label transport case with lithium battery label.

Accessories

Design
Plexiglas bar (15 cm x 4.5 cm) with upward curved end possessing a port for positioning at 60° or 90° relative to the sample level the DIVING-PAM-II fiber optics. Mounted to the curved end is a 5.5 cm x 7.5 cm (W x H) sample clip consisting of a Plexiglas plate (lower part) and an aluminum frame open to the top (upper part). Featuring a 10 cm long plastic grip with button for triggering measurements via a 1.5 m trigger cable. Including a 1 m long tubular net with zipper to keep together trigger cable and fiber optics. With holder to for spectrometer MINI-SPEC
Dimensions
25 cm x 4.5 cm x 21 cm (L x W x H)
Weight
380 g
Design
Holder made of grey PVC, equipped with 3 rubber bands and hooks to be attached to creviced surface (e.g. of coral); nylon screws for distance adjustment
Dimensions
6 cm x 6 cm x 2.5 cm (L x W x H)
Weight
95 g
Design
Two plastic halves with magnets, one with sliding shutter made of spring steel and seat for the adapter DIVING-DA. The other halve with seats for additional magnets. Inner sides of both halves covered with black fabric. Including 4 additional magnets.
Dimensions
Diameter 30 mm, height 28 mm
Weight
10 g
Design
Three clips made of white plastic with gasket contact areas and sliding shutter for light-tight closure.
Dimensions
Diameter 3.2 cm, length 8 cm
Weight
6.5 g

Active diameter 2 mm, length 1.5 m. Both ends with adapter which fits to the fiber port of the DIVING-PAM-II and the various accessories for fiber positioning of the DIVING-PAM-II system.