Two add-ons convert the PAM-2500 chlorophyll fluorometer into a highly mobile field station, equipped with the options that a normal PC offers and working for hours independently of line current.
• A touch-screen ultra-mobile personal computer (UMPC; for instance the Acer Iconia W3) equipped with a high capacity battery. The UMPC is placed on top of the PAM-2500 fluorometer using a special metal box strapped to the fluorometer’s central unit.
• An external battery (12 V, 2.1 Ah) for the PAM-2500 chlorophyll fluorometer. An automatic charger for this battery is available.
All parts required for long-term battery-powered operation of the PAM-2500 chlorophyll fluorometer fit into the PAM-2500 carrying bag which is supplied with the basic system.
Information on ambient light and temperature conditions is indispensable for field investigation. Therefore, we have devised the leaf-clip 2030-B which measures by a mini-quantum sensor the photosynthetically active radiation (PAR) impinging on the leaf under investigation. In addition, the 2030-B leaf-clip records the leaf temperature with a NiCr-Ni thermocouple.
To facilitate studies under field conditions, fluorescence measurements can be triggered by pushing the control button of the leaf-clip holder 2030-B. A tripod (ST-2101A) is available for positioning of the leaf-clip 2030-B but also the mini quantum/temperature-sensor 2060-M or the Arabidopsis leaf clip 2060-B.
The holder positions the PAM-2500 fiberoptics on bulky samples. Combinable with the 2060-M Mini Quantum/Temp.-Sensor, to measure temperature and impinging PAR of the surface area investigated.
Aluminum clip with small measuring area designed to position small leaves below the fiberoptics of the PAM-2500. When combined with the 2060-M Mini Quantum/Temp.-Sensor, PAR on sample level and lower leaf temperature is recorded.
Precise mini quantum and temperature sensors usable independently or in conjunction with the 2060-B Arabidopsis Leaf Clip or the 2060-A Fiberoptics Holder for Surfaces.
The fiberoptics adapter 90º can be attached to the leaf clips 2030-B and 2060-B to position the PAM-2500 fiberoptics at 90° angle relative to leaf plane.
The DLC-8 leaves clips permit dark-acclimation of small leaf areas in the field. The tip of the PAM-2500 fiber optics fits exactly in the DLC-8 port. With the fiber tip inserted, the sliding shutter of the DLC-8 can be opened so that F0 and FM level fluorescence can be measured without interference of ambient light.
For measurements of attached leaves with the fiber optics, we have developed the leaf positioning device, DUAL-BA. The device includes a fiber optics holder, a magnetic end piece for the fiber optics and a right-angled metal sheet that sticks to the end piece by magnetic force. The metal sheet positions the leaf in front of the fiber optics end.
The suspension cuvette includes a 400 μl sample compartment made of stainless steel with PVC exterior. The cuvette is equipped with a 7 mm fiberoptics window adapter, an injection port for Hamilton syringes, and nozzles for connecting an external flow-through water-bath for temperature control.
The device is equipped with a specially modified stirrer plate to center and hold the KS-2500 suspension cuvette. The MKS-2500 stirrer comes with a Perspex base plate with stand bar for mounting fiberoptics on top of cuvette.